利用Dualex葉綠素儀測定水稻葉片氮素

更新時間：2022-05-06　點擊量：2543

Dualex是一款源自于法國國家科學院 (CNRS)及巴黎第十一大學技術，由奧地利PESSL公司生產（原法國Force-A公司）開發的新型多功能葉片測量儀。它可同時準確測量葉片的葉綠素含量、葉片表層的類黃酮和花青素含量，適用于植物生理學和農學（如水稻葉綠素濃度，玉米氮素狀況，葡萄藤等）相關研究。

其測量對象可以是單子葉植物，雙子葉植物或多年生植物。這款設備簡單易用，可進行實時和非破壞性測量。由于不需要校準標定和事先的樣品制備，測量工作可在實驗室或現場完成。此外，該設備在各種溫度的和環境光照條件下均可正常使用。系統采用光學傳感器可進行簡單、快速、無損測量葉片中的葉綠素、多酚以及花青素。

Dualex便攜式智能氣象-葉綠素-花青素-黃酮醇測量儀

摘要

準確估計和監測作物氮素有助于及時診斷，并為肥料管理提供必要的技術支持。在中國東南部進行了四個試驗，涉及三個品種和六個氮（N）處理，以比較兩個葉夾測量儀Dualex 4 Scientific+與SPAD測量儀測量氮營養相關指標的能力。結果表明，與SPAD值相比，Chl對單位葉面積葉綠素的監測精度更高，在高葉綠素濃度狀態下不出現飽和現象。黃酮類化合物（Flav）具有早期診斷水稻氮素營養狀況的優勢（與SPAD值相比大約一天）。作為一種可靠的氮素營養診斷指標，與傳統的SPAD方法相比，該方法提高了估算精度。另一個Dualex值也獲得了良好的監測結果。Flav與缺氮呈正相關，在低RMSE的情況下，分別與穗分化期和孕穗期較高的R2呈正相關；SPAD值與氮缺乏呈負相關。因此，基于Flav的氮肥施用模式為早稻提供了一種氮肥施用途徑，尤其是在穗分化和孕穗期。

關鍵詞：類黃酮；氮營養；氮調節；糧食產量；氮平衡指數

A Comparative Assessment of Measures of Leaf Nitrogen in Rice Using Two Leaf-Clip Meters

Abstract

Accurate estimation and monitoring of crop nitrogen can assist in timely diagnosis and facilitate necessary technical support for fertilizer management. Four experiments, involving three c*rs and six nitrogen (N) treatments, were conducted in southeast China to compare the two leaf-clip meters (Dualex 4 Scientific+, Force-A, Orasy, France; Soil and Plant Analyzer Development (SPAD) meter, Minolta Camera Co., Osaka, Japan) for their ability to measure nitrogen nutrient-related indicators. The results indicated that Chl had a better monitoring accuracy for chlorophyll in per unit leaf area as compared to SPAD value, and there was no saturation to appear under high leaf chlorophyll concentration status. Flavonoids (Flav) presented the advantage of early diagnosis of rice N nutrition status (about one day as compared to SPAD value). As a reliable N nutrient diagnosis indicator, it also improved the estimation accuracy compared with the classical SPAD-based method. The other Dualex value also obtained good monitoring results. Flav was positively correlated with N deficiency, and with higher R2 in panicle initiation and booting stages with low RMSE, respectively; whereas SPAD value was negatively correlated with nitrogen deficiency. Therefore, the Flav-based nitrogen application model was found to provide an early rice nitrogen fertilizer application approach, especially in the panicle initiation and booting stages.

Keywords: flavonoid; nitrogen nutrient; nitrogen regulation; grain yield; nitrogen balance index